# Experiments **URL:** https://www.evolver.bio/c/experiment/14.md [Latest](https://www.evolver.bio/latest.md) · [Categories](https://www.evolver.bio/categories.md) --- ## [About the Experiments category](https://www.evolver.bio/t/about-the-experiments-category/24)
**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\ **Replies:** 0
--- ## [10% Bleach - Where to buy? Best suppliers (Europe)?](https://www.evolver.bio/t/10-bleach-where-to-buy-best-suppliers-europe/609)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 5\ **Last updated:** [February 6, 2026, 8:45am UTC](https://www.evolver.bio/t/10-bleach-where-to-buy-best-suppliers-europe/609 "2026-02-06T08:45:10Z")
Hi everyone, Where do you guys buy bleach (sodium hypochlorite)? Buying ready-to-use 10% bleach is easier, but more expensive of course. Could some people perhaps share where they buy their bleach and how they prepare… --- ## [Temperature limitations](https://www.evolver.bio/t/temperature-limitations/325)
**Author:** [@Anush](https://www.evolver.bio/u/Anush)\ **Replies:** 5\ **Last updated:** [January 16, 2026, 8:00pm UTC](https://www.evolver.bio/t/temperature-limitations/325 "2026-01-16T20:00:37Z")
Hello eVOLVER experts, I am trying to set up some experiments with eVOLVER and would need it to work at 58-60 degrees Celsius. Has someone operated at these temperatures before? The main paper says the max T would be 45… --- ## [ePACE Pump Mapping](https://www.evolver.bio/t/epace-pump-mapping/608)
**Author:** [@jayzoellin](https://www.evolver.bio/u/jayzoellin)\ **Replies:** 3\ **Last updated:** [September 19, 2025, 10:43pm UTC](https://www.evolver.bio/t/epace-pump-mapping/608 "2025-09-19T22:43:26Z")
Hi all, Thanks for the excellent documentation and the amazing device We’re setting up ePACE on a newly acquired eVOLVER unit. Our configuration: 16 low-flow (~1 mL/min) and 32 high-flow (~45 mL/min) pumps. We plan to r… --- ## [OD reading noise issues](https://www.evolver.bio/t/od-reading-noise-issues/607)
**Author:** [@elib](https://www.evolver.bio/u/elib)\ **Replies:** 1\ **Last updated:** [September 17, 2025, 2:41am UTC](https://www.evolver.bio/t/od-reading-noise-issues/607 "2025-09-17T02:41:39Z")
Hello, I have been having serious issues with noise during growth curve experiments with E. coli. Below is an example. Raw data is available here: https://drive.google.com/file/d/1VwbU3IDWpBykSehsTw3WW09jp8U2NLuP/view?us… --- ## [3D print 5-port vial caps in ABS?](https://www.evolver.bio/t/3d-print-5-port-vial-caps-in-abs/572)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 1\ **Last updated:** [June 1, 2025, 3:56pm UTC](https://www.evolver.bio/t/3d-print-5-port-vial-caps-in-abs/572 "2025-06-01T15:56:06Z")
Hi everyone, Instead of nylon PA-12 to print these 5-port vial caps (\[hardware/Smart Sleeve/vial\_cap/5\_and\_7\_port\_nylon\_tube/vial\_cap\_5ports+sample.STL at master · FYNCH-BIO/hardware · GitHub\]), is it OK to use ABS as m… --- ## [OD Calibration NaN problem](https://www.evolver.bio/t/od-calibration-nan-problem/202)
**Author:** [@AdamLyon](https://www.evolver.bio/u/AdamLyon)\ **Replies:** 3\ **Last updated:** [April 25, 2025, 2:59pm UTC](https://www.evolver.bio/t/od-calibration-nan-problem/202 "2025-04-25T14:59:48Z")
I’ve encountered a problem in retrieving my OD calibration file I write the line: py calibration/calibrate.py -a 192.168.1.2 -n lyoOD820 -f fitOD -t linear -p od\_90, od\_135 And I get an output with lists of lists… --- ## [Iterate OD calibration when refreshing the media](https://www.evolver.bio/t/iterate-od-calibration-when-refreshing-the-media/504)
**Author:** [@sunachi](https://www.evolver.bio/u/sunachi)\ **Replies:** 0\ **Last updated:** [December 23, 2023, 9:32am UTC](https://www.evolver.bio/t/iterate-od-calibration-when-refreshing-the-media/504 "2023-12-23T09:32:23Z")
Hi guys! I’m planning to run a turbidostat which is going to be a long period (for weeks). I am wandering that when the prepared media is fully used and refresh the media to the one of same ingredients, should I recali… --- ## [About the Volume of the required media](https://www.evolver.bio/t/about-the-volume-of-the-required-media/330)
**Author:** [@sunachi](https://www.evolver.bio/u/sunachi)\ **Replies:** 6\ **Last updated:** [June 11, 2023, 1:03pm UTC](https://www.evolver.bio/t/about-the-volume-of-the-required-media/330 "2023-06-11T13:03:15Z")
Hi, eVOLVER experts I am now setting some experiments, and found that I need 25 L for almost 5 days experiments ! (20 mL for culture volumes, 1.5 h for Avg.doubleing time, 16 for the number of cultures) I calculated th… --- ## [Adding the 2nd set of pump to the turbidiostat experiment](https://www.evolver.bio/t/adding-the-2nd-set-of-pump-to-the-turbidiostat-experiment/461)
**Author:** [@Christian](https://www.evolver.bio/u/Christian)\ **Replies:** 5\ **Last updated:** [May 31, 2023, 11:11pm UTC](https://www.evolver.bio/t/adding-the-2nd-set-of-pump-to-the-turbidiostat-experiment/461 "2023-05-31T23:11:41Z")
Hey there, I was wondering about how to add the 2nd set of pumps to the turbiduistat experiment. Specifically: Pumpset one: “Normal medium in-pump” (As for the normal experiment) Pumpset two: “Normal out-pump” - I wan… --- ## [Noisy readings despite OK calibration](https://www.evolver.bio/t/noisy-readings-despite-ok-calibration/430)
**Author:** [@Quentin](https://www.evolver.bio/u/Quentin)\ **Replies:** 4\ **Last updated:** [January 30, 2023, 11:49am UTC](https://www.evolver.bio/t/noisy-readings-despite-ok-calibration/430 "2023-01-30T11:49:28Z")
Hello everyone, I am having an issue with one of the position of the eVOLVER. I am working with fission yeats, growing them between 0.2 and 0.4 OD. The data I am showing were obtained using this calibration: NB: it … --- ## [Could i run ePACE system on eVOLVER?](https://www.evolver.bio/t/could-i-run-epace-system-on-evolver/426)
**Author:** [@Baelab](https://www.evolver.bio/u/Baelab)\ **Replies:** 8\ **Last updated:** [December 27, 2022, 8:50pm UTC](https://www.evolver.bio/t/could-i-run-epace-system-on-evolver/426 "2022-12-27T20:50:15Z")
Hi, I am now planning a ePACE system setup using eVOLVER. However, there is one question in this regard. The PACE system requires two major types of bio-reactors. The first is a ‘MAIN reactor’ that causes infection… --- ## [Aerobic Batch Experiment Setup](https://www.evolver.bio/t/aerobic-batch-experiment-setup/381)
**Author:** [@hoyt.k](https://www.evolver.bio/u/hoyt.k)\ **Replies:** 1\ **Last updated:** [August 31, 2022, 3:50pm UTC](https://www.evolver.bio/t/aerobic-batch-experiment-setup/381 "2022-08-31T15:50:19Z")
Hi! Has anyone grown aerobic organisms in eVOLVER in batch (no pumps, no feed lines)? If so, were you able to get good O2 transfer into the media? What caps/septa did you use? How about stir rate and media volume v. … --- ## [Bubbles being introduced to media tubing](https://www.evolver.bio/t/bubbles-being-introduced-to-media-tubing/297)
**Author:** [@ericlyall](https://www.evolver.bio/u/ericlyall)\ **Replies:** 3\ **Last updated:** [April 30, 2021, 6:00pm UTC](https://www.evolver.bio/t/bubbles-being-introduced-to-media-tubing/297 "2021-04-30T18:00:36Z")
I’m getting a lot of bubbles in my tubing. Some background: I fill the vials using the evolver tubing by pumping for around a minute. There will be no bubbles at this time. I’ll inoculate with a small amount of bacteri… --- ## [Recommendations for Septas and Needles?](https://www.evolver.bio/t/recommendations-for-septas-and-needles/59)
**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\ **Replies:** 16\ **Last updated:** [March 6, 2021, 12:17am UTC](https://www.evolver.bio/t/recommendations-for-septas-and-needles/59 "2021-03-06T00:17:46Z")
Question: Does anyone have any recommendations for extra long needles and septa for applications requiring gas exchange, using the typical 40mL chemglass borosilicate vessel? Answer: We’ve recently been 3D printing ny… --- ## [OD Calibrations](https://www.evolver.bio/t/od-calibrations/257)
**Author:** [@ericlyall](https://www.evolver.bio/u/ericlyall)\ **Replies:** 3\ **Last updated:** [January 28, 2021, 3:11pm UTC](https://www.evolver.bio/t/od-calibrations/257 "2021-01-28T15:11:56Z")
A few questions about the OD calibrations: Are these calibration curves good / in line with the calibrations most users get? I’m getting a few r squared values of .92 and .95, and the rest close to .99. Most concerni… --- ## [Sterilisation of nylon caps](https://www.evolver.bio/t/sterilisation-of-nylon-caps/256)
**Author:** [@viktoriiagross](https://www.evolver.bio/u/viktoriiagross)\ **Replies:** 1\ **Last updated:** [January 22, 2021, 3:24pm UTC](https://www.evolver.bio/t/sterilisation-of-nylon-caps/256 "2021-01-22T15:24:08Z")
Hi everyone! I am Viktoriia, a PhD student, and I am just starting to work with Imane El Meouche with eVOLVER. I have a question about means of sterilisation of vial caps. Is there some way other than autoclave to ste… --- ## [Units of stir speed](https://www.evolver.bio/t/units-of-stir-speed/239)
**Author:** [@trash](https://www.evolver.bio/u/trash)\ **Replies:** 4\ **Last updated:** [December 31, 2020, 5:27pm UTC](https://www.evolver.bio/t/units-of-stir-speed/239 "2020-12-31T17:27:15Z")
What is the unit of stir speed? I see that some examples given are 8, 10, 12 in the code but there is no indication of units. I’d also be happy to make a github issue to fix this. --- ## [Unexpected double dilution](https://www.evolver.bio/t/unexpected-double-dilution/226)
**Author:** [@danigarcia](https://www.evolver.bio/u/danigarcia)\ **Replies:** 9\ **Last updated:** [December 7, 2020, 5:03pm UTC](https://www.evolver.bio/t/unexpected-double-dilution/226 "2020-12-07T17:03:01Z")
Hi there! I’m running a turbidostat experiment with an OD range between 0.3 and 0.4. Lately, some of the vials diluted below 0.25 on most of the dilution points, as you can see in the example image. I investigated th… --- ## [OD oscillating, even with no sample](https://www.evolver.bio/t/od-oscillating-even-with-no-sample/209)
**Author:** [@hoyt.k](https://www.evolver.bio/u/hoyt.k)\ **Replies:** 4\ **Last updated:** [September 30, 2020, 7:07pm UTC](https://www.evolver.bio/t/od-oscillating-even-with-no-sample/209 "2020-09-30T19:07:20Z")
Hello, Has anyone encountered oscillations in their OD readings, even in empty smart sleeves? Any ideas why? We tried covering the sleeves with foil, in case it was caused by ambient light, but that did not stop the os… --- ## [Initial OD Calibration](https://www.evolver.bio/t/initial-od-calibration/198)
**Author:** [@hoyt.k](https://www.evolver.bio/u/hoyt.k)\ **Replies:** 1\ **Last updated:** [August 6, 2020, 3:55pm UTC](https://www.evolver.bio/t/initial-od-calibration/198 "2020-08-06T15:55:09Z")
Hi! I am planning out my first OD calibration. I plan to use cells directly, and wanted to confirm a few other pieces of my workflow. Below are my questions: It looks like the current eVOLVER code will calibrate both … --- ## [Where can I order additional nylon caps?](https://www.evolver.bio/t/where-can-i-order-additional-nylon-caps/81)
**Author:** [@melosbor](https://www.evolver.bio/u/melosbor)\ **Replies:** 4\ **Last updated:** [July 28, 2020, 8:58pm UTC](https://www.evolver.bio/t/where-can-i-order-additional-nylon-caps/81 "2020-07-28T20:58:04Z")
Can I order additional 3D printed vial caps from FynchBio? --- ## [Where or how is OD blank info stored?](https://www.evolver.bio/t/where-or-how-is-od-blank-info-stored/182)
**Author:** [@danigarcia](https://www.evolver.bio/u/danigarcia)\ **Replies:** 9\ **Last updated:** [June 23, 2020, 11:08am UTC](https://www.evolver.bio/t/where-or-how-is-od-blank-info-stored/182 "2020-06-23T11:08:57Z")
I’m starting a turbidostat experiment with 4 vials and I will be adding the rest every few days. For this, I wanted to be able to blank the OD of those vials just before adding them to the experiment instead of doing it … --- ## [Issues with OD/temp measurements](https://www.evolver.bio/t/issues-with-od-temp-measurements/129)
**Author:** [@langevin.am](https://www.evolver.bio/u/langevin.am)\ **Replies:** 23\ **Last updated:** [October 10, 2019, 9:02pm UTC](https://www.evolver.bio/t/issues-with-od-temp-measurements/129 "2019-10-10T21:02:24Z")
We’ve had a couple issues pop up during our last experiment(s): \[OD1\] Two vial that were blanked, but rose immediately (within a couple minutes) to an OD of 0.3 despite not being inoculated. However, upon looking at t… --- ## [Making Bottles and Media Splitters](https://www.evolver.bio/t/making-bottles-and-media-splitters/63)
**Author:** [@cmancuso](https://www.evolver.bio/u/cmancuso)\ **Replies:** 9\ **Last updated:** [October 8, 2019, 11:29am UTC](https://www.evolver.bio/t/making-bottles-and-media-splitters/63 "2019-10-08T11:29:57Z")
While some groups like to use large carboys for media, I find that individual bottles makes media preparation easier. It’s also essential for experiments with many different media types. Here’s how I make media bottles f… --- ## [Some vials stop back-diluting mid experiment](https://www.evolver.bio/t/some-vials-stop-back-diluting-mid-experiment/121)
**Author:** [@mabrams](https://www.evolver.bio/u/mabrams)\ **Replies:** 5\ **Last updated:** [July 30, 2019, 1:11am UTC](https://www.evolver.bio/t/some-vials-stop-back-diluting-mid-experiment/121 "2019-07-30T01:11:20Z")
Hi, I’ve been running an experiment for ~850 hours, and for the last ~100 hours about half of the vials stopped back-diluting. I had not touched the machine for over 50 hours at that point. I’ve turned off-and-on the … --- ## [Verifying Calibrations - Poor Accuracy](https://www.evolver.bio/t/verifying-calibrations-poor-accuracy/112)
**Author:** [@santibk](https://www.evolver.bio/u/santibk)\ **Replies:** 3\ **Last updated:** [July 17, 2019, 6:05pm UTC](https://www.evolver.bio/t/verifying-calibrations-poor-accuracy/112 "2019-07-17T18:05:20Z")
Hi all, I have been continuing someone else’s work using our evolver unit and when they left, they documented that several of the sleeves either do not work altogether or have significant issues taking OD measurements. … --- ## [Mid-experiment Noise in OD measurements](https://www.evolver.bio/t/mid-experiment-noise-in-od-measurements/107)
**Author:** [@langevin.am](https://www.evolver.bio/u/langevin.am)\ **Replies:** 3\ **Last updated:** [July 2, 2019, 3:02pm UTC](https://www.evolver.bio/t/mid-experiment-noise-in-od-measurements/107 "2019-07-02T15:02:04Z")
I ran a longer multi-day experiment and most of the vials preformed well, but something strange happened with vial 15 right after the 24 hour mark. All vials run with LB + Tween20 0.5% (v/v) and different concentrations… --- ## [Using nylon caps to determine culture volumes](https://www.evolver.bio/t/using-nylon-caps-to-determine-culture-volumes/91)
**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\ **Replies:** 5\ **Last updated:** [June 5, 2019, 8:36pm UTC](https://www.evolver.bio/t/using-nylon-caps-to-determine-culture-volumes/91 "2019-06-05T20:36:56Z")
EDIT: Using syringe needles is recommended now (https://www.evolver.bio/t/recommendations-for-septas-and-needles/59) Summary In this tutorial, I hope to teach how to build your own Smart Sleeve vials and determine cultu… --- ## [How to visualize my OD calibration curve](https://www.evolver.bio/t/how-to-visualize-my-od-calibration-curve/98)
**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\ **Replies:** 0\ **Last updated:** [May 21, 2019, 3:48pm UTC](https://www.evolver.bio/t/how-to-visualize-my-od-calibration-curve/98 "2019-05-21T15:48:15Z")
The eVOLVER currently holds all the raw data for temperature and OD calibration. To view the graphs, the eVOLVER is connected and the data is graphed with a small Python script. The system is designed this way better man… [Next page](https://www.evolver.bio/c/experiment/14.md?page=1)