# Software

**URL:** https://www.evolver.bio/c/software/8.md

[Latest](https://www.evolver.bio/latest.md) · [Categories](https://www.evolver.bio/categories.md)

---

## [About the Software category](https://www.evolver.bio/t/about-the-software-category/18)

<div class="topic-metadata">

**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\
**Replies:** 0

</div>

All code is located in the Github Repositories: eVOLVER Code (Raspberry Pi, Python) Arduino Code (C++) Data Processing Unit (Python) eVOLVER GUI (NodeJS) A basic user would only need to interact with the DPU code.

---

## [Playing around with code to learn](https://www.evolver.bio/t/playing-around-with-code-to-learn/600)

<div class="topic-metadata">

**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\
**Replies:** 5\
**Last updated:** [February 13, 2026, 7:05pm UTC](https://www.evolver.bio/t/playing-around-with-code-to-learn/600 "2026-02-13T19:05:59Z")

</div>

Hi all, Thus far I managed to get to the point where I was able to connect to the eVOLVER using command line stuff. I’m playing around with water just to get a grip of Python language (I’m a complete noob) and I’m tryin…

---

## [Issues compiling evolver-electron on Ubuntu 22.04](https://www.evolver.bio/t/issues-compiling-evolver-electron-on-ubuntu-22-04/612)

<div class="topic-metadata">

**Author:** [@cns99](https://www.evolver.bio/u/cns99)\
**Replies:** 0\
**Last updated:** [October 22, 2025, 8:15pm UTC](https://www.evolver.bio/t/issues-compiling-evolver-electron-on-ubuntu-22-04/612 "2025-10-22T20:15:03Z")

</div>

Hi all, I’m attempting to install evolver-electron on a lab Ubuntu 22.04 x86\_64 system. I had no issues installing the dpu and running the eVOLVER CLI, but since the evolver-electron release .deb and AppImages are only …

---

## [DPU installation issues](https://www.evolver.bio/t/dpu-installation-issues/611)

<div class="topic-metadata">

**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\
**Replies:** 2\
**Last updated:** [October 21, 2025, 10:25am UTC](https://www.evolver.bio/t/dpu-installation-issues/611 "2025-10-21T10:25:24Z")

</div>

Hi, I am trying to install the DPU on a new (dedicated) laptop (Windows 11). It seems like the command is not able to properly install poetry using the line of code mentioned on your Wiki page. Any advice please?

---

## [DPU Installing Error](https://www.evolver.bio/t/dpu-installing-error/584)

<div class="topic-metadata">

**Author:** [@Rita.Chen](https://www.evolver.bio/u/Rita.Chen)\
**Replies:** 1\
**Last updated:** [August 1, 2025, 8:27am UTC](https://www.evolver.bio/t/dpu-installing-error/584 "2025-08-01T08:27:41Z")

</div>

I encountered an error while attempting to install DPU version 2.0.2, following the instructions provided on the wiki. Could anyone assist me in resolving this issue?

---

## [eVOLVER Connection Error](https://www.evolver.bio/t/evolver-connection-error/581)

<div class="topic-metadata">

**Author:** [@cb\_goat](https://www.evolver.bio/u/cb_goat)\
**Replies:** 6\
**Last updated:** [July 28, 2025, 1:55pm UTC](https://www.evolver.bio/t/evolver-connection-error/581 "2025-07-28T13:55:40Z")

</div>

Hello, I am having trouble connecting the eVOLVER to the server. When I turn on the system, I get a “eVOLVER Connection Error” on the display window. I am suspecting it may have to do with the RS-485 board, but I did ch…

---

## [How and where do I reconfigure pump assignments?](https://www.evolver.bio/t/how-and-where-do-i-reconfigure-pump-assignments/596)

<div class="topic-metadata">

**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\
**Replies:** 1\
**Last updated:** [July 18, 2025, 9:09pm UTC](https://www.evolver.bio/t/how-and-where-do-i-reconfigure-pump-assignments/596 "2025-07-18T21:09:54Z")

</div>

Dear all, How and where do I re-assign pump IDs in order to get to the following configuration? (see images) How are the pumps numbered in a default setting? I read somewhere that the slow pumps can only be used via ‘…

---

## [Electron GUI not loading after fresh installation](https://www.evolver.bio/t/electron-gui-not-loading-after-fresh-installation/519)

<div class="topic-metadata">

**Author:** [@unwaege](https://www.evolver.bio/u/unwaege)\
**Replies:** 7\
**Last updated:** [July 15, 2025, 8:05pm UTC](https://www.evolver.bio/t/electron-gui-not-loading-after-fresh-installation/519 "2025-07-15T20:05:16Z")

</div>

Our chemostat computer had to be factory reset, so we had to rebuild the eVOLVER software, and encountered issues with the electron GUI. After installing the .dmg file for our Mac as instructed by the wiki, the eVOLVER a…

---

## [Stuck with DPU installation](https://www.evolver.bio/t/stuck-with-dpu-installation/589)

<div class="topic-metadata">

**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\
**Replies:** 3\
**Last updated:** [July 10, 2025, 5:23pm UTC](https://www.evolver.bio/t/stuck-with-dpu-installation/589 "2025-07-10T17:23:40Z")

</div>

Hi all, I’m stuck at step 9: DPU Installation | eVOLVER What should I do?

---

## [Trouble installing DPU](https://www.evolver.bio/t/trouble-installing-dpu/529)

<div class="topic-metadata">

**Author:** [@Hanna\_T](https://www.evolver.bio/u/Hanna_T)\
**Replies:** 3\
**Last updated:** [July 6, 2025, 9:16pm UTC](https://www.evolver.bio/t/trouble-installing-dpu/529 "2025-07-06T21:16:15Z")

</div>

Hi, I am trying to install DPU for our new eVOLVER and have run into a problem following the instructions on the wiki. I downloaded the 2.0.2 release from GitHub but this happens when I try to use poetry to install depe…

---

## [Trouble updating OD calibration](https://www.evolver.bio/t/trouble-updating-od-calibration/539)

<div class="topic-metadata">

**Author:** [@antonio.volpini](https://www.evolver.bio/u/antonio.volpini)\
**Replies:** 3\
**Last updated:** [October 11, 2024, 1:38pm UTC](https://www.evolver.bio/t/trouble-updating-od-calibration/539 "2024-10-11T13:38:26Z")

</div>

Hi, I recently rebuilt the evolver with the updated 5.2 vial holder design, and then performed temp and OD calibrations afterward. I have been able to use the updated temp calibration, but have not been able to load the …

---

## [Trouble running an experiment via the GUI](https://www.evolver.bio/t/trouble-running-an-experiment-via-the-gui/531)

<div class="topic-metadata">

**Author:** [@Hanna\_T](https://www.evolver.bio/u/Hanna_T)\
**Replies:** 1\
**Last updated:** [August 15, 2024, 1:20pm UTC](https://www.evolver.bio/t/trouble-running-an-experiment-via-the-gui/531 "2024-08-15T13:20:12Z")

</div>

Hi, I’ve encountered another problem (I’m new to eVOLVER, so it probably won’t be the last one). I did all the calibrations via the GUI, it worked (I hope), but I’m unable to start an experiment. The folder for it gets…

---

## [Experiments will not run at set temperatures](https://www.evolver.bio/t/experiments-will-not-run-at-set-temperatures/527)

<div class="topic-metadata">

**Author:** [@JimBreaking](https://www.evolver.bio/u/JimBreaking)\
**Replies:** 8\
**Last updated:** [August 7, 2024, 1:15pm UTC](https://www.evolver.bio/t/experiments-will-not-run-at-set-temperatures/527 "2024-08-07T13:15:36Z")

</div>

Greetings all. We have just set up our evolver and I am trying to do some testing. I set up experiments with either all vials at 37 C or 25 C and the evolver will only run at 30 C. The experimental parameters are show…

---

## [Raspi image not working](https://www.evolver.bio/t/raspi-image-not-working/523)

<div class="topic-metadata">

**Author:** [@jmgb](https://www.evolver.bio/u/jmgb)\
**Replies:** 4\
**Last updated:** [July 10, 2024, 2:15pm UTC](https://www.evolver.bio/t/raspi-image-not-working/523 "2024-07-10T14:15:00Z")

</div>

I used the raspi image from here: Tried with multiple sd cards and when booting up it gets stuck on the fynchbio image. Thanks,

---

## [Calibration Errors](https://www.evolver.bio/t/calibration-errors/509)

<div class="topic-metadata">

**Author:** [@William](https://www.evolver.bio/u/William)\
**Replies:** 1\
**Last updated:** [February 9, 2024, 10:38pm UTC](https://www.evolver.bio/t/calibration-errors/509 "2024-02-09T22:38:47Z")

</div>

I am trying to calibrate the eVOLVER and have run into an issue when calibrating the OD and the temperature, but not the pumps, through the GUI. I have attached the error message I receive when I go to run the calibratio…

---

## [Can I apply a new OD calibration to old data?](https://www.evolver.bio/t/can-i-apply-a-new-od-calibration-to-old-data/291)

<div class="topic-metadata">

**Author:** [@hoyt.k](https://www.evolver.bio/u/hoyt.k)\
**Replies:** 5\
**Last updated:** [December 1, 2023, 10:20am UTC](https://www.evolver.bio/t/can-i-apply-a-new-od-calibration-to-old-data/291 "2023-12-01T10:20:29Z")

</div>

Hello, Is there a way to apply a new OD calibration fit to raw OD readings from old experiments? It seems like a new fit could be applied to the raw OD values in an old experiment folder, but I can’t quite figure out h…

---

## [Sigmoid fit - OD Calibration](https://www.evolver.bio/t/sigmoid-fit-od-calibration/481)

<div class="topic-metadata">

**Author:** [@eduarda](https://www.evolver.bio/u/eduarda)\
**Replies:** 1\
**Last updated:** [October 5, 2023, 12:56pm UTC](https://www.evolver.bio/t/sigmoid-fit-od-calibration/481 "2023-10-05T12:56:28Z")

</div>

Hello Everyone! I’ve seen lots of discussion on how to make better fits when calibrating OD but it seems to me as though maybe the sigmoid curve isn’t the best way to to fit the points. Why was is chosen? Has anyone tri…

---

## [Some changes in the code resulting in error messeages](https://www.evolver.bio/t/some-changes-in-the-code-resulting-in-error-messeages/480)

<div class="topic-metadata">

**Author:** [@Christian](https://www.evolver.bio/u/Christian)\
**Replies:** 3\
**Last updated:** [September 29, 2023, 11:59am UTC](https://www.evolver.bio/t/some-changes-in-the-code-resulting-in-error-messeages/480 "2023-09-29T11:59:45Z")

</div>

Hey, I did some changes to include more pumps and changed the mode of the Tstat. Now I ran into this: “eVOLVER - \[CRITICAL\] - exception Some errors were detected ! Line #3 (got 6 columns instead of 4) stopped the expe…

---

## [Updating the eVOLVER server](https://www.evolver.bio/t/updating-the-evolver-server/69)

<div class="topic-metadata">

**Author:** [@heinsz](https://www.evolver.bio/u/heinsz)\
**Replies:** 11\
**Last updated:** [September 27, 2023, 1:04pm UTC](https://www.evolver.bio/t/updating-the-evolver-server/69 "2023-09-27T13:04:20Z")

</div>

This is how I update the code on eVOLVER units when we have software updates. I plan to make deployment scripts in the future to streamline this, but if you only have one or two eVOLVERs to update this process is fairly …

---

## [Issues with Populating Active Calibrations in Experimental Setup Dashboard](https://www.evolver.bio/t/issues-with-populating-active-calibrations-in-experimental-setup-dashboard/478)

<div class="topic-metadata">

**Author:** [@esouth](https://www.evolver.bio/u/esouth)\
**Replies:** 3\
**Last updated:** [September 1, 2023, 3:04pm UTC](https://www.evolver.bio/t/issues-with-populating-active-calibrations-in-experimental-setup-dashboard/478 "2023-09-01T15:04:17Z")

</div>

Hello, I’m a new user reviving an eVOLVER setup from 2019, and I’m currently working with DPU v2.0.1 and Electron v2.0.0. I’m facing an issue with the Experimental Setup Dashboard. When I try to select any “Active Calib…

---

## [Vial-wise Temperature Calibration](https://www.evolver.bio/t/vial-wise-temperature-calibration/475)

<div class="topic-metadata">

**Author:** [@rmemon](https://www.evolver.bio/u/rmemon)\
**Replies:** 2\
**Last updated:** [August 27, 2023, 12:02pm UTC](https://www.evolver.bio/t/vial-wise-temperature-calibration/475 "2023-08-27T12:02:35Z")

</div>

Hello, I’m starting the temperature calibrations for the eVOLVER unit we got from FynchBio. For each temperature level, I noticed it takes about 30 minutes (minimum, after allowing the thermometer to equilibrate after p…

---

## [Uncaught Javascript Exception during Temperature Calibration](https://www.evolver.bio/t/uncaught-javascript-exception-during-temperature-calibration/474)

<div class="topic-metadata">

**Author:** [@rmemon](https://www.evolver.bio/u/rmemon)\
**Replies:** 1\
**Last updated:** [August 25, 2023, 3:25pm UTC](https://www.evolver.bio/t/uncaught-javascript-exception-during-temperature-calibration/474 "2023-08-25T15:25:38Z")

</div>

I am using an M2 Mac mini as my dedicated eVOLVER computer. When I run the temperature calibration and I click the edit pencil button after completing the measurements, I got this error: Uncaught Exception: Error:…

---

## [Switching steering off during OD measurement](https://www.evolver.bio/t/switching-steering-off-during-od-measurement/470)

<div class="topic-metadata">

**Author:** [@konst\_ac](https://www.evolver.bio/u/konst_ac)\
**Replies:** 1\
**Last updated:** [July 27, 2023, 6:44pm UTC](https://www.evolver.bio/t/switching-steering-off-during-od-measurement/470 "2023-07-27T18:44:45Z")

</div>

Hello! We would like to stop steering every time the OD is measured to get a more precise result. From what we understood the OD measurements occur atomaticaly on the server without any instructions from the client, so …

---

## [Questions about time\_in variable](https://www.evolver.bio/t/questions-about-time-in-variable/468)

<div class="topic-metadata">

**Author:** [@Christian](https://www.evolver.bio/u/Christian)\
**Replies:** 2\
**Last updated:** [July 5, 2023, 6:04pm UTC](https://www.evolver.bio/t/questions-about-time-in-variable/468 "2023-07-05T18:04:18Z")

</div>

Hei, I was wondering about the code for calculating the time in the pump commands in the t-stat experiment. The code is as follows: time\_in = - (np.log(lower\_thresh\[x\]/average\_OD)\*VOLUME)/flow\_rate\[x\] Where the next …

---

## [DPU installation issue - Exit code 1](https://www.evolver.bio/t/dpu-installation-issue-exit-code-1/414)

<div class="topic-metadata">

**Author:** [@karolbuda](https://www.evolver.bio/u/karolbuda)\
**Replies:** 16\
**Last updated:** [June 26, 2023, 4:38pm UTC](https://www.evolver.bio/t/dpu-installation-issue-exit-code-1/414 "2023-06-26T16:38:13Z")

</div>

Hi all, We have purchased a new Windows computer for the eVOLVER and are reinstalling the software. I ran into this issue: The virtual environment is running python 3.11 and both pip and setuptools have been checked…

---

## [Evolver SocketIO python versions](https://www.evolver.bio/t/evolver-socketio-python-versions/439)

<div class="topic-metadata">

**Author:** [@vhu9644](https://www.evolver.bio/u/vhu9644)\
**Replies:** 7\
**Last updated:** [June 2, 2023, 4:27pm UTC](https://www.evolver.bio/t/evolver-socketio-python-versions/439 "2023-06-02T16:27:07Z")

</div>

Hello, So we’ve been running a altered version of the evolver and dpu code, and I had a few questions: Why is the dpu code using socketio-client instead of python-socketio? Is there any push to update the evolver pyth…

---

## [New RPi 3 Model B+](https://www.evolver.bio/t/new-rpi-3-model-b/456)

<div class="topic-metadata">

**Author:** [@kawidney](https://www.evolver.bio/u/kawidney)\
**Replies:** 7\
**Last updated:** [May 17, 2023, 4:36pm UTC](https://www.evolver.bio/t/new-rpi-3-model-b/456 "2023-05-17T16:36:37Z")

</div>

Hi, our raspberry pi seemed to have been damaged when we had a spill. We have a new one to replace it, but when I plug it in with the old micro sd, nothing appears to happen (the red power light comes on, and the green l…

---

## [White touchscreen](https://www.evolver.bio/t/white-touchscreen/197)

<div class="topic-metadata">

**Author:** [@danigarcia](https://www.evolver.bio/u/danigarcia)\
**Replies:** 7\
**Last updated:** [January 2, 2023, 4:35pm UTC](https://www.evolver.bio/t/white-touchscreen/197 "2023-01-02T16:35:07Z")

</div>

Hi! Some weeks ago I was running a two weeks turbidostat with the eVOLVER and, at some point (\>12 days), the screen turned completely white. I completely stopped the experiment control script in the computer and restarte…

---

## [Why am I receiving a "no vials selected" error message when I do have vials selected?](https://www.evolver.bio/t/why-am-i-receiving-a-no-vials-selected-error-message-when-i-do-have-vials-selected/398)

<div class="topic-metadata">

**Author:** [@jerrydinan](https://www.evolver.bio/u/jerrydinan)\
**Replies:** 2\
**Last updated:** [September 20, 2022, 11:01pm UTC](https://www.evolver.bio/t/why-am-i-receiving-a-no-vials-selected-error-message-when-i-do-have-vials-selected/398 "2022-09-20T23:01:05Z")

</div>

Hi everyone, This is my first post here, so I hope this is the correct category for my question. I am trying to manually run the pumps for a selection of vials. I first selected the vials on the DPU, then clicked th…

---

## [eVOLVER Wiki + Software Release 2.0.0](https://www.evolver.bio/t/evolver-wiki-software-release-2-0-0/383)

<div class="topic-metadata">

**Author:** [@heinsz](https://www.evolver.bio/u/heinsz)\
**Replies:** 25\
**Last updated:** [September 16, 2022, 2:41am UTC](https://www.evolver.bio/t/evolver-wiki-software-release-2-0-0/383 "2022-09-16T02:41:15Z")

</div>

Hi everyone! We have a couple of new developments in software/documentation that I would like to outline here. eVOLVER Documentation Wiki First, I’d like to announce that we now have an official open-source eVOLVER wik…

[Next page](https://www.evolver.bio/c/software/8.md?page=1)
