# Uncategorized **URL:** https://www.evolver.bio/c/uncategorized/1.md [Latest](https://www.evolver.bio/latest.md) · [Categories](https://www.evolver.bio/categories.md) --- ## [Welcome to Discourse](https://www.evolver.bio/t/welcome-to-discourse/7)
**Author:** [@system](https://www.evolver.bio/u/system)\ **Replies:** 0\ **Last updated:** [March 14, 2019, 6:56pm UTC](https://www.evolver.bio/t/welcome-to-discourse/7 "2019-03-14T18:56:30Z")
Welcome to the eVOLVER Forums. The purpose of these forums is to help facilitate sharing of protocols, experimental ideas, and new hardware developments for the eVOLVER system (https://www.nature.com/articles/nbt.4151). … --- ## [Calibration Files in eVOLVER](https://www.evolver.bio/t/calibration-files-in-evolver/108)
**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\ **Replies:** 9\ **Last updated:** [June 6, 2025, 7:11pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108 "2025-06-06T19:11:39Z")
Question: Where are the calibration files saved? We’ve poked through all the scripts and searched everywhere on the computer. There should be some .txt files somewhere, right? Any idea where they would be saved or wher… --- ## [LEDs on RIGHT PWM will not turn off completely and RPI Not Submitting Commands](https://www.evolver.bio/t/leds-on-right-pwm-will-not-turn-off-completely-and-rpi-not-submitting-commands/282)
**Author:** [@maraxen](https://www.evolver.bio/u/maraxen)\ **Replies:** 12\ **Last updated:** [April 11, 2025, 7:24pm UTC](https://www.evolver.bio/t/leds-on-right-pwm-will-not-turn-off-completely-and-rpi-not-submitting-commands/282 "2025-04-11T19:24:29Z")
I am installing blue LEDs on our eVOLVER for optogenetic applications. To do this, I added a light variable to the conf.yml on the RPI and updated the Arduino handling a PWM in SA slot 8. I was able to get control from c… --- ## [Pump Calibration](https://www.evolver.bio/t/pump-calibration/486)
**Author:** [@William](https://www.evolver.bio/u/William)\ **Replies:** 0\ **Last updated:** [October 31, 2023, 3:36pm UTC](https://www.evolver.bio/t/pump-calibration/486 "2023-10-31T15:36:34Z")
I have been using the eVOLVER without the pumps for some time, but am now looking to expand the work to chemostat. I am trying to calibrate the pumps and have the most recent version of the software installed which inclu… --- ## [General improvements using eVOLVER as a turbidostat](https://www.evolver.bio/t/general-improvements-using-evolver-as-a-turbidostat/298)
**Author:** [@dcoudreuse](https://www.evolver.bio/u/dcoudreuse)\ **Replies:** 9\ **Last updated:** [July 26, 2023, 10:42am UTC](https://www.evolver.bio/t/general-improvements-using-evolver-as-a-turbidostat/298 "2023-07-26T10:42:54Z")
Dear all, Since we have acquired our eVOLVERs, we have been working a fair bit on adapting them to our needs and changing a number of procedures that seemed to help the reproducibility and robustness of our experiments.… --- ## [OD calibration issues - signal maxed out?](https://www.evolver.bio/t/od-calibration-issues-signal-maxed-out/447)
**Author:** [@kawidney](https://www.evolver.bio/u/kawidney)\ **Replies:** 8\ **Last updated:** [April 8, 2023, 12:55pm UTC](https://www.evolver.bio/t/od-calibration-issues-signal-maxed-out/447 "2023-04-08T12:55:08Z")
Hi, I was wondering if anyone has run into an issue where it seems like the light detectors are being maxed out? I’m having trouble with our OD calibrations, and it the signal from the sensors stays relatively constant r… --- ## [eVOLVER modifications made at the Brazilian Center for Research in Energy and Materials](https://www.evolver.bio/t/evolver-modifications-made-at-the-brazilian-center-for-research-in-energy-and-materials/436)
**Author:** [@eduarda](https://www.evolver.bio/u/eduarda)\ **Replies:** 3\ **Last updated:** [March 20, 2023, 11:52am UTC](https://www.evolver.bio/t/evolver-modifications-made-at-the-brazilian-center-for-research-in-energy-and-materials/436 "2023-03-20T11:52:16Z")
Hello! My name is Eduarda, I’m an intern in the Brazilian Center for Research in Energy and Materials (CNPEM). The Engineering division here at the Center has been studying the eVOLVER framework and building one from sc… --- ## [Resistor Network for the Motherboard](https://www.evolver.bio/t/resistor-network-for-the-motherboard/388)
**Author:** [@eduarda](https://www.evolver.bio/u/eduarda)\ **Replies:** 5\ **Last updated:** [October 30, 2022, 8:24pm UTC](https://www.evolver.bio/t/resistor-network-for-the-motherboard/388 "2022-10-30T20:24:29Z")
Hello! The motherboard has RP1,…, RP16 where I assume resistor networks are put in the connectors. What resistance would the networks be? Thank you in advance. --- ## [Cannot run server through commandline](https://www.evolver.bio/t/cannot-run-server-through-commandline/402)
**Author:** [@kawidney](https://www.evolver.bio/u/kawidney)\ **Replies:** 14\ **Last updated:** [October 19, 2022, 1:43am UTC](https://www.evolver.bio/t/cannot-run-server-through-commandline/402 "2022-10-19T01:43:38Z")
Hi, I’m having issues getting the server to run correctly after having updated. Here is the error code I get. I also cannot get the GUI to work for looking at my experiment - I keep getting a Java error Environment: Re… --- ## [Adding sensors (pH and DO)](https://www.evolver.bio/t/adding-sensors-ph-and-do/353)
**Author:** [@eduarda](https://www.evolver.bio/u/eduarda)\ **Replies:** 1\ **Last updated:** [February 18, 2022, 6:49pm UTC](https://www.evolver.bio/t/adding-sensors-ph-and-do/353 "2022-02-18T18:49:48Z")
Hello!! I would like to add a pH sensor and a dissolved oxygen sensor to my smart sleeve. Has anyone tried this before? Does anyone have any insight in this matter? If you’ve added a sensor to your smart sleeve before,… --- ## [Black touchscreen on eVOLVER](https://www.evolver.bio/t/black-touchscreen-on-evolver/313)
**Author:** [@ericlyall](https://www.evolver.bio/u/ericlyall)\ **Replies:** 1\ **Last updated:** [July 6, 2021, 1:34pm UTC](https://www.evolver.bio/t/black-touchscreen-on-evolver/313 "2021-07-06T13:34:33Z")
I was starting an experiment with my eVOLVER, and the screen on the evolver started to flicker with black lines going horizontally across the screen. It eventually went to fully black, but is still backlit when turned on… --- ## [Pump in from multiple sources](https://www.evolver.bio/t/pump-in-from-multiple-sources/308)
**Author:** [@sophie9499](https://www.evolver.bio/u/sophie9499)\ **Replies:** 3\ **Last updated:** [June 23, 2021, 3:06pm UTC](https://www.evolver.bio/t/pump-in-from-multiple-sources/308 "2021-06-23T15:06:47Z")
Hi people, I’m trying to pump in from two sources (different amount) into one vial so I wonder if it’s possible and if you’ve got it to work before, I’d really appreciate any suggestions and advice! Thanks! --- ## [DIY low cost version of eVOLVER](https://www.evolver.bio/t/diy-low-cost-version-of-evolver/287)
**Author:** [@trbs](https://www.evolver.bio/u/trbs)\ **Replies:** 2\ **Last updated:** [April 3, 2021, 6:04pm UTC](https://www.evolver.bio/t/diy-low-cost-version-of-evolver/287 "2021-04-03T18:04:39Z")
Hi! I was wondering whether you would ever publish a full list of parts by supplier and make available those parts that are custom made (i.e. printed circuit boards and microfluidic chip), leaving the assembly to the us… --- ## [12V Power Supply Connection to Motherboard Melted](https://www.evolver.bio/t/12v-power-supply-connection-to-motherboard-melted/263)
**Author:** [@maraxen](https://www.evolver.bio/u/maraxen)\ **Replies:** 10\ **Last updated:** [February 12, 2021, 7:59pm UTC](https://www.evolver.bio/t/12v-power-supply-connection-to-motherboard-melted/263 "2021-02-12T19:59:30Z")
The title sounds more dramatic than reality (although no less concerning), but the intermittent drops we had been experiencing with the 12V power supply appear to have some connection to this melted rubber over the wire … --- ## [12V Power Supply Intermittent Drops?](https://www.evolver.bio/t/12v-power-supply-intermittent-drops/258)
**Author:** [@maraxen](https://www.evolver.bio/u/maraxen)\ **Replies:** 2\ **Last updated:** [January 28, 2021, 10:35pm UTC](https://www.evolver.bio/t/12v-power-supply-intermittent-drops/258 "2021-01-28T22:35:09Z")
The 12V-rated LEDs on our eVOLVER will slightly dim intermittently for a breif period before brightness is restored. Looking at the fans with no vial in the sleeves, they also have the same intermittent periods where the… --- ## [Clarification on Current Power Supply Set-Up](https://www.evolver.bio/t/clarification-on-current-power-supply-set-up/252)
**Author:** [@maraxen](https://www.evolver.bio/u/maraxen)\ **Replies:** 3\ **Last updated:** [January 14, 2021, 8:43pm UTC](https://www.evolver.bio/t/clarification-on-current-power-supply-set-up/252 "2021-01-14T20:43:20Z")
Our lab has 12V blue LEDs currently linked to the 5V and I am thinking of putting the PWM that controls them on the 24V lead so we have access to the full range of brightness, but I am a little confused on what I would n… --- ## [Issues with OD calibration](https://www.evolver.bio/t/issues-with-od-calibration/250)
**Author:** [@AdamLyon](https://www.evolver.bio/u/AdamLyon)\ **Replies:** 2\ **Last updated:** [January 14, 2021, 7:29pm UTC](https://www.evolver.bio/t/issues-with-od-calibration/250 "2021-01-14T19:29:09Z")
Hi everyone, I recently did an OD calibration. I had multiple samples with the same OD however, the curves don’t look as expected. Below is the result of a sigmoid fit with od90 and below that is the sigmoid fit for od1… --- ## [Would full fan spin speed be okay (for bacteria)?](https://www.evolver.bio/t/would-full-fan-spin-speed-be-okay-for-bacteria/233)
**Author:** [@maraxen](https://www.evolver.bio/u/maraxen)\ **Replies:** 1\ **Last updated:** [December 23, 2020, 5:51pm UTC](https://www.evolver.bio/t/would-full-fan-spin-speed-be-okay-for-bacteria/233 "2020-12-23T17:51:05Z")
A spill damaged one of the fans on our eVOLVER and we are working on getting a trained replacement but in the interim, we have replaced it with a standard fan from the manufacturer and moved the 3D printed magnet chassis… --- ## [A simple CLI for running eVOLVER scripts](https://www.evolver.bio/t/a-simple-cli-for-running-evolver-scripts/234)
**Author:** [@maraxen](https://www.evolver.bio/u/maraxen)\ **Replies:** 0\ **Last updated:** [December 8, 2020, 7:06pm UTC](https://www.evolver.bio/t/a-simple-cli-for-running-evolver-scripts/234 "2020-12-08T19:06:18Z")
I have put together a bash script to improve ease of use for my lab. It automates the commands and has run all the components you can call through. I also compiled a batch script for Windows users, which I have not had a… --- ## [Feedback on Our eVOLVER](https://www.evolver.bio/t/feedback-on-our-evolver/230)
**Author:** [@maraxen](https://www.evolver.bio/u/maraxen)\ **Replies:** 2\ **Last updated:** [November 18, 2020, 4:54pm UTC](https://www.evolver.bio/t/feedback-on-our-evolver/230 "2020-11-18T16:54:33Z")
Hi everyone! I’m Marielle, a Research Assistant and I’ve been working on getting the eVOLVER running and ready for experiments ove the past months took thorough notes on my process. We thought it might be useful to provi… --- ## [Where are SA slots specified?](https://www.evolver.bio/t/where-are-sa-slots-specified/204)
**Author:** [@Max\_Schubert](https://www.evolver.bio/u/Max_Schubert)\ **Replies:** 4\ **Last updated:** [August 31, 2020, 6:59pm UTC](https://www.evolver.bio/t/where-are-sa-slots-specified/204 "2020-08-31T18:59:14Z")
I’m curious about applications where I’m adding additional LEDs and/or diodes to the eVOLVER setup. If I’m changing the vial board so that “light” is on pin pair 6… where do I update the info such that “light” commands m… --- ## [Issues re-installing evolver scripts](https://www.evolver.bio/t/issues-re-installing-evolver-scripts/134)
**Author:** [@Max\_Schubert](https://www.evolver.bio/u/Max_Schubert)\ **Replies:** 2\ **Last updated:** [August 28, 2019, 12:36pm UTC](https://www.evolver.bio/t/issues-re-installing-evolver-scripts/134 "2019-08-28T12:36:29Z")
My hard disk crapped out after everything was perfectly set up, so I’m putting things pack together again with the new drive. I followed the setup commands at https://github.com/FYNCH-BIO/dpu including but i’m confused… --- ## [New topics missing from the forums](https://www.evolver.bio/t/new-topics-missing-from-the-forums/26)
**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\ **Replies:** 2\ **Last updated:** [March 16, 2019, 10:01pm UTC](https://www.evolver.bio/t/new-topics-missing-from-the-forums/26 "2019-03-16T22:01:50Z")
Wanted to start a discussion/thread about adding new topics to either distribute work to people interested in helping out. I will continue to add and modify this post as ideas come up. Topics of interest include: Ster…