# Latest **URL:** https://www.evolver.bio/latest.md [Latest](https://www.evolver.bio/latest.md) · [Categories](https://www.evolver.bio/categories.md) --- ## [Welcome to Discourse](https://www.evolver.bio/t/welcome-to-discourse/7)
**Author:** [@system](https://www.evolver.bio/u/system)\ **Replies:** 0\ **Last updated:** [March 14, 2019, 6:56pm UTC](https://www.evolver.bio/t/welcome-to-discourse/7 "2019-03-14T18:56:30Z")
Welcome to the eVOLVER Forums. The purpose of these forums is to help facilitate sharing of protocols, experimental ideas, and new hardware developments for the eVOLVER system (https://www.nature.com/articles/nbt.4151). … --- ## [Looking for Smart Sleeve Acrylic Plate Files (Parts #9, #10, #11)](https://www.evolver.bio/t/looking-for-smart-sleeve-acrylic-plate-files-parts-9-10-11/625)
**Author:** [@JinHyeongKim](https://www.evolver.bio/u/JinHyeongKim)\ **Replies:** 1\ **Last updated:** [June 25, 2026, 11:38pm UTC](https://www.evolver.bio/t/looking-for-smart-sleeve-acrylic-plate-files-parts-9-10-11/625 "2026-06-25T23:38:46Z")
Hello, I am currently building a Smart Sleeve and I am trying to identify the acrylic parts shown in the assembly guide (parts #9, #10, and #11). I have reviewed the Smart Sleeve build guide and searched through the Gi… --- ## [Where can I find the STL file for the magnet holder?](https://www.evolver.bio/t/where-can-i-find-the-stl-file-for-the-magnet-holder/623)
**Author:** [@JinHyeongKim](https://www.evolver.bio/u/JinHyeongKim)\ **Replies:** 1\ **Last updated:** [June 17, 2026, 2:55pm UTC](https://www.evolver.bio/t/where-can-i-find-the-stl-file-for-the-magnet-holder/623 "2026-06-17T14:55:25Z")
Hello, I am currently building an eVOLVER system and I’m looking for the STL file for the magnet holder used in the Smart Sleeve stirrer assembly. I checked the GitHub repositories and available 3D files, but I could n… --- ## [Pump 6 continuously pumping WITHOUT command](https://www.evolver.bio/t/pump-6-continuously-pumping-without-command/621)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 15\ **Last updated:** [June 2, 2026, 5:56pm UTC](https://www.evolver.bio/t/pump-6-continuously-pumping-without-command/621 "2026-06-02T17:56:22Z")
Hi all, Today I wanted to initiate an experiment, but somehow something went haywire. I started filling Tstats using the GUI as I always do to prep the machine (we run our own scripts using the terminal thereafter). Ho… --- ## [Exposure of eVOLVER to UV light in a BSC II](https://www.evolver.bio/t/exposure-of-evolver-to-uv-light-in-a-bsc-ii/622)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 0\ **Last updated:** [May 13, 2026, 5:52am UTC](https://www.evolver.bio/t/exposure-of-evolver-to-uv-light-in-a-bsc-ii/622 "2026-05-13T05:52:23Z")
Dear all, Is there anyone else who has their eVOLVER set up inside a biosafety cabinet? If so, do you turn on the UV lamp for (additional) decontamination after experiments? We have the black acrylic splash box that c… --- ## [Pumps not responding to commands (i.e. not turning on)](https://www.evolver.bio/t/pumps-not-responding-to-commands-i-e-not-turning-on/617)
**Author:** [@ptse1121](https://www.evolver.bio/u/ptse1121)\ **Replies:** 21\ **Last updated:** [March 4, 2026, 11:49pm UTC](https://www.evolver.bio/t/pumps-not-responding-to-commands-i-e-not-turning-on/617 "2026-03-04T23:49:49Z")
Hi all, I’m currently trying to set up an eVOLVER. I have been able to complete temperature and OD calibrations, but I cannot seem to get the pumps to respond to any commands. I have tried starting the pumps from the pu… --- ## [Playing around with code to learn](https://www.evolver.bio/t/playing-around-with-code-to-learn/600)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 5\ **Last updated:** [February 13, 2026, 7:05pm UTC](https://www.evolver.bio/t/playing-around-with-code-to-learn/600 "2026-02-13T19:05:59Z")
Hi all, Thus far I managed to get to the point where I was able to connect to the eVOLVER using command line stuff. I’m playing around with water just to get a grip of Python language (I’m a complete noob) and I’m tryin… --- ## [10% Bleach - Where to buy? Best suppliers (Europe)?](https://www.evolver.bio/t/10-bleach-where-to-buy-best-suppliers-europe/609)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 5\ **Last updated:** [February 6, 2026, 8:45am UTC](https://www.evolver.bio/t/10-bleach-where-to-buy-best-suppliers-europe/609 "2026-02-06T08:45:10Z")
Hi everyone, Where do you guys buy bleach (sodium hypochlorite)? Buying ready-to-use 10% bleach is easier, but more expensive of course. Could some people perhaps share where they buy their bleach and how they prepare… --- ## [Particulates in media lines](https://www.evolver.bio/t/particulates-in-media-lines/615)
**Author:** [@elib](https://www.evolver.bio/u/elib)\ **Replies:** 8\ **Last updated:** [January 30, 2026, 7:03pm UTC](https://www.evolver.bio/t/particulates-in-media-lines/615 "2026-01-30T19:03:56Z")
Hello, I’ve had a persistent problem with small particles of unknown material being introduced into vials through the pump system, which interferes with OD readings and potentially contaminates our cultures. These partic… --- ## [Temperature limitations](https://www.evolver.bio/t/temperature-limitations/325)
**Author:** [@Anush](https://www.evolver.bio/u/Anush)\ **Replies:** 5\ **Last updated:** [January 16, 2026, 8:00pm UTC](https://www.evolver.bio/t/temperature-limitations/325 "2026-01-16T20:00:37Z")
Hello eVOLVER experts, I am trying to set up some experiments with eVOLVER and would need it to work at 58-60 degrees Celsius. Has someone operated at these temperatures before? The main paper says the max T would be 45… --- ## [Issues compiling evolver-electron on Ubuntu 22.04](https://www.evolver.bio/t/issues-compiling-evolver-electron-on-ubuntu-22-04/612)
**Author:** [@cns99](https://www.evolver.bio/u/cns99)\ **Replies:** 0\ **Last updated:** [October 22, 2025, 8:15pm UTC](https://www.evolver.bio/t/issues-compiling-evolver-electron-on-ubuntu-22-04/612 "2025-10-22T20:15:03Z")
Hi all, I’m attempting to install evolver-electron on a lab Ubuntu 22.04 x86\_64 system. I had no issues installing the dpu and running the eVOLVER CLI, but since the evolver-electron release .deb and AppImages are only … --- ## [DPU installation issues](https://www.evolver.bio/t/dpu-installation-issues/611)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 2\ **Last updated:** [October 21, 2025, 10:25am UTC](https://www.evolver.bio/t/dpu-installation-issues/611 "2025-10-21T10:25:24Z")
Hi, I am trying to install the DPU on a new (dedicated) laptop (Windows 11). It seems like the command is not able to properly install poetry using the line of code mentioned on your Wiki page. Any advice please? --- ## [ePACE Pump Mapping](https://www.evolver.bio/t/epace-pump-mapping/608)
**Author:** [@jayzoellin](https://www.evolver.bio/u/jayzoellin)\ **Replies:** 3\ **Last updated:** [September 19, 2025, 10:43pm UTC](https://www.evolver.bio/t/epace-pump-mapping/608 "2025-09-19T22:43:26Z")
Hi all, Thanks for the excellent documentation and the amazing device We’re setting up ePACE on a newly acquired eVOLVER unit. Our configuration: 16 low-flow (~1 mL/min) and 32 high-flow (~45 mL/min) pumps. We plan to r… --- ## [OD reading noise issues](https://www.evolver.bio/t/od-reading-noise-issues/607)
**Author:** [@elib](https://www.evolver.bio/u/elib)\ **Replies:** 1\ **Last updated:** [September 17, 2025, 2:41am UTC](https://www.evolver.bio/t/od-reading-noise-issues/607 "2025-09-17T02:41:39Z")
Hello, I have been having serious issues with noise during growth curve experiments with E. coli. Below is an example. Raw data is available here: https://drive.google.com/file/d/1VwbU3IDWpBykSehsTw3WW09jp8U2NLuP/view?us… --- ## [Corroded Ribbon Cable Port](https://www.evolver.bio/t/corroded-ribbon-cable-port/605)
**Author:** [@apemaraj](https://www.evolver.bio/u/apemaraj)\ **Replies:** 1\ **Last updated:** [September 17, 2025, 2:37am UTC](https://www.evolver.bio/t/corroded-ribbon-cable-port/605 "2025-09-17T02:37:14Z")
Hi all, Recently one of our eVOVLERs had a major leak resulting in fluid entering the ribbon cable ports for two vials. We have tested the smart sleeves and ribbon cables and found them to be working fine, but the port … --- ## [How to fix the magnet to the fan](https://www.evolver.bio/t/how-to-fix-the-magnet-to-the-fan/606)
**Author:** [@JinHyeongKim](https://www.evolver.bio/u/JinHyeongKim)\ **Replies:** 1\ **Last updated:** [September 17, 2025, 2:33am UTC](https://www.evolver.bio/t/how-to-fix-the-magnet-to-the-fan/606 "2025-09-17T02:33:44Z")
Hello. I just want to fix the magnet on the fan, so can I fix it with a bond? There’s nothing much in the 3D data of Github. --- ## [Motherboard configuration and pwm, adc board configuration](https://www.evolver.bio/t/motherboard-configuration-and-pwm-adc-board-configuration/604)
**Author:** [@JinHyeongKim](https://www.evolver.bio/u/JinHyeongKim)\ **Replies:** 2\ **Last updated:** [September 8, 2025, 9:56am UTC](https://www.evolver.bio/t/motherboard-configuration-and-pwm-adc-board-configuration/604 "2025-09-08T09:56:22Z")
Hello. I want to experiment in Korea, so I can’t purchase the product, so I want to organize the experiment directly through open source. My question is, I would like to ask if it is possible to experiment with only 3… --- ## [Peristaltic Pumps Not Turning On](https://www.evolver.bio/t/peristaltic-pumps-not-turning-on/594)
**Author:** [@apemaraj](https://www.evolver.bio/u/apemaraj)\ **Replies:** 2\ **Last updated:** [September 4, 2025, 8:58pm UTC](https://www.evolver.bio/t/peristaltic-pumps-not-turning-on/594 "2025-09-04T20:58:35Z")
Hi all, I am working on getting an eVOLVER experiment set up and am having some trouble setting up the peristaltic pumps purchased from FynchBio. To my knowledge we purchased this eVOVLER a while ago, but have not yet u… --- ## [DPU Installing Error](https://www.evolver.bio/t/dpu-installing-error/584)
**Author:** [@Rita.Chen](https://www.evolver.bio/u/Rita.Chen)\ **Replies:** 1\ **Last updated:** [August 1, 2025, 8:27am UTC](https://www.evolver.bio/t/dpu-installing-error/584 "2025-08-01T08:27:41Z")
I encountered an error while attempting to install DPU version 2.0.2, following the instructions provided on the wiki. Could anyone assist me in resolving this issue? --- ## [Software Installation and Startup Questions](https://www.evolver.bio/t/software-installation-and-startup-questions/493)
**Author:** [@Nate\_Borders](https://www.evolver.bio/u/Nate_Borders)\ **Replies:** 5\ **Last updated:** [July 31, 2025, 3:26pm UTC](https://www.evolver.bio/t/software-installation-and-startup-questions/493 "2025-07-31T15:26:22Z")
Post here if you have questions about installing software for and starting up a min-eVOLVER. Wiki guide here. --- ## [Building a Smart Sleeve for Continuous Culture (40 mL)](https://www.evolver.bio/t/building-a-smart-sleeve-for-continuous-culture-40-ml/30)
**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\ **Replies:** 5\ **Last updated:** [July 31, 2025, 2:15pm UTC](https://www.evolver.bio/t/building-a-smart-sleeve-for-continuous-culture-40-ml/30 "2025-07-31T14:15:55Z")
Background eVOLVER hardware is primarily divided between the Smart Sleeves and Motherboard. The Motherboard is where all the sensitive control hardware is, whereas the Smart Sleeve is nice modular way of integrating all… --- ## [Pump tubing replacement](https://www.evolver.bio/t/pump-tubing-replacement/585)
**Author:** [@elib](https://www.evolver.bio/u/elib)\ **Replies:** 1\ **Last updated:** [July 30, 2025, 3:28pm UTC](https://www.evolver.bio/t/pump-tubing-replacement/585 "2025-07-30T15:28:57Z")
Hello, the tubing on one of my pumps tore (see pic) and I’m wondering how to go about replacing. First, what are the specs on the tubing so I can order a replacement? Second, what is the best way to disassemble the pu… --- ## [Technical Drawing for Evolver Splash Shield](https://www.evolver.bio/t/technical-drawing-for-evolver-splash-shield/444)
**Author:** [@vhu9644](https://www.evolver.bio/u/vhu9644)\ **Replies:** 4\ **Last updated:** [July 30, 2025, 2:53pm UTC](https://www.evolver.bio/t/technical-drawing-for-evolver-splash-shield/444 "2025-07-30T14:53:48Z")
Hello, I wanted to make a sheet metal shield for the evolver that should block out nearby sources of electrical and IR noise, as well as serving as a splash shield. Are there technical drawings for the placement of the … --- ## [eVOLVER Connection Error](https://www.evolver.bio/t/evolver-connection-error/581)
**Author:** [@cb\_goat](https://www.evolver.bio/u/cb_goat)\ **Replies:** 6\ **Last updated:** [July 28, 2025, 1:55pm UTC](https://www.evolver.bio/t/evolver-connection-error/581 "2025-07-28T13:55:40Z")
Hello, I am having trouble connecting the eVOLVER to the server. When I turn on the system, I get a “eVOLVER Connection Error” on the display window. I am suspecting it may have to do with the RS-485 board, but I did ch… --- ## [Temperature Stability issues](https://www.evolver.bio/t/temperature-stability-issues/597)
**Author:** [@elib](https://www.evolver.bio/u/elib)\ **Replies:** 5\ **Last updated:** [July 25, 2025, 12:57am UTC](https://www.evolver.bio/t/temperature-stability-issues/597 "2025-07-25T00:57:43Z")
Hello, I am having serious issues with temperature stability with my eVOLVER unit. During temperature calibration at room temperature per the wiki instructions, the water vials are not reaching an equilibrium temperature… --- ## [How and where do I reconfigure pump assignments?](https://www.evolver.bio/t/how-and-where-do-i-reconfigure-pump-assignments/596)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 1\ **Last updated:** [July 18, 2025, 9:09pm UTC](https://www.evolver.bio/t/how-and-where-do-i-reconfigure-pump-assignments/596 "2025-07-18T21:09:54Z")
Dear all, How and where do I re-assign pump IDs in order to get to the following configuration? (see images) How are the pumps numbered in a default setting? I read somewhere that the slow pumps can only be used via ‘… --- ## [Biosafety cabinet Class A2 for eVOLVER](https://www.evolver.bio/t/biosafety-cabinet-class-a2-for-evolver/595)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 1\ **Last updated:** [July 17, 2025, 3:22pm UTC](https://www.evolver.bio/t/biosafety-cabinet-class-a2-for-evolver/595 "2025-07-17T15:22:57Z")
Dear all, Anyone who has placed their eVOLVER inside a BSC class A2? I am sourcing for providers that deliver BSC’s that have a larger working depth than 60 cm: I would want to place the vial platform inside such that … --- ## [Electron GUI not loading after fresh installation](https://www.evolver.bio/t/electron-gui-not-loading-after-fresh-installation/519)
**Author:** [@unwaege](https://www.evolver.bio/u/unwaege)\ **Replies:** 7\ **Last updated:** [July 15, 2025, 8:05pm UTC](https://www.evolver.bio/t/electron-gui-not-loading-after-fresh-installation/519 "2025-07-15T20:05:16Z")
Our chemostat computer had to be factory reset, so we had to rebuild the eVOLVER software, and encountered issues with the electron GUI. After installing the .dmg file for our Mac as instructed by the wiki, the eVOLVER a… --- ## [Stuck with DPU installation](https://www.evolver.bio/t/stuck-with-dpu-installation/589)
**Author:** [@KevinDM](https://www.evolver.bio/u/KevinDM)\ **Replies:** 3\ **Last updated:** [July 10, 2025, 5:23pm UTC](https://www.evolver.bio/t/stuck-with-dpu-installation/589 "2025-07-10T17:23:40Z")
Hi all, I’m stuck at step 9: DPU Installation | eVOLVER What should I do? --- ## [Tutorial - Configuring Local Router and Computer for Basic eVOLVER Use](https://www.evolver.bio/t/tutorial-configuring-local-router-and-computer-for-basic-evolver-use/71)
**Author:** [@bgwong](https://www.evolver.bio/u/bgwong)\ **Replies:** 17\ **Last updated:** [July 10, 2025, 11:43am UTC](https://www.evolver.bio/t/tutorial-configuring-local-router-and-computer-for-basic-evolver-use/71 "2025-07-10T11:43:02Z")
Summary In this tutorial, we will go over how to set up a local NETGEAR router to properly connect to eVOLVER. In this setup, the eVOLVER is not directly connected to the internet. If you desire the eVOLVER to be conne… [Next page](https://www.evolver.bio/latest.md?page=1)