# Calibration Files in eVOLVER **URL:** \ **Category:** Uncategorized\ **Created:** [July 1, 2019, 8:30pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108 "2019-07-01T20:30:13Z")\ **Posts on this page:** 10\ **Page:** 1
**Author:** ![bgwong](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/bgwong/32/262_2.png) [@bgwong](https://www.evolver.bio/u/bgwong)\ **Post date:** [July 1, 2019, 8:30pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/1 "2019-07-01T20:30:13Z")
**Question:** Where are the calibration files saved? We’ve poked through all the scripts and searched everywhere on the computer. There should be some .txt files somewhere, right? Any idea where they would be saved or where the custom\_script file would be pulling from? **Response:** _ **Selecting Active Calibration File for the Experiment** _ So all the calibration files are saved on the eVOLVER and the custom script grabs the calibration files from the eVOLVER before every experiment. You can set what the active file is from the the setup screen on either the touchscreen or desktop application. ![image](https://canada1.discourse-cdn.com/flex035/uploads/evolver/original/1X/6ca8a7dd90f63d21a2fb82355909fadf86f34e76.png) _custom\_script.py_ automatically saves the calibration file from whatever is active. _ **Generating a New Calibration File** _ To create a new calibration file, please navigate to the appropriate calibration GUI (temp or OD). There, follow the instructions on the GUI in order to properly calibrate the sensors. I plan on adding additional instructions later. Completion of the calibration saves the **raw data** on the eVOLVER. To interpret the sensor data, run a python script following this [readme](https://github.com/FYNCH-BIO/dpu). Specifically the " Run calibration code (after the raw values have been logged on the eVOLVER)" section. ---
**Author:** ![ericlyall](https://avatars.discourse-cdn.com/v4/letter/e/d2c977/32.png) [@ericlyall](https://www.evolver.bio/u/ericlyall)\ **Post date:** [January 20, 2021, 12:39am UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/2 "2021-01-20T00:39:17Z")
When executing the calibration script on python, can you explain a bit more on what to enter for -p? Does it matter what I type there? Or is it just a variable name for graphing? We will be calibrating with OD\_600. ![image](https://canada1.discourse-cdn.com/flex035/uploads/evolver/original/1X/f922266cba59c93daf253b38f7cb3f278319c134.png) ---
**Author:** ![bgwong](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/bgwong/32/262_2.png) [@bgwong](https://www.evolver.bio/u/bgwong)\ **Post date:** [January 20, 2021, 12:56am UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/3 "2021-01-20T00:56:33Z")
The od\_90 or od\_135 refers to the angle the photodiode is to the LED, not the wavelength of light. You have to type in od\_90 or od\_135 to calibrate those respective photodiodes. Below is a thread on why we have two photodiodes. > [@OD measurements with two photodiodes?](https://www.evolver.bio/t/od-measurements-with-two-photodiodes/99): > > Summary I’m looking for some advice on how to properly do calibrations with two variables. Background I’m trying to integrate hardware configuration for OD measurements to improve the consistency and dynamic range of the sensors. I figured adding a different sensor at a different LED-diode offset would do the trick. I did a simple, rough experiment and collected measurements off of 90 degree and 135 degree offsets with increasing amount of milk (to approximate scattering from cells). Heres t… ---
**Author:** ![mrebolleda](https://avatars.discourse-cdn.com/v4/letter/m/d9b06d/32.png) [@mrebolleda](https://www.evolver.bio/u/mrebolleda)\ **Post date:** [April 15, 2021, 2:26pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/4 "2021-04-15T14:26:02Z")
Hi, I am María and I am just starting to use the eVOLVER. I used the GUI to make a new O.D. calibration, but I cannot find it in the setup dashboard. How can I load the right calibration? Thank you. ---
**Author:** ![bgwong](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/bgwong/32/262_2.png) [@bgwong](https://www.evolver.bio/u/bgwong)\ **Post date:** [April 15, 2021, 3:10pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/5 "2021-04-15T15:10:19Z")
Hi Maria, Excited for you to start using eVOLVER! Did you run the calibrate.py scripts? > After you run the calibration OD GUI, it saves the raw data in a file on the eVOLVER. It doesn’t get fitted until you run the calibrate.py script from your lab computer. It is a bit non-intuitive right now, but we’re working on integrating that with the GUI. Best, Brandon ---
**Author:** ![mrebolleda](https://avatars.discourse-cdn.com/v4/letter/m/d9b06d/32.png) [@mrebolleda](https://www.evolver.bio/u/mrebolleda)\ **Post date:** [April 15, 2021, 3:45pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/6 "2021-04-15T15:45:02Z")
Hi Brandon, Thank you for the fast reply. We tried to run the calibrate.py but it gets stuck in the message “connected to eVOLVER as client” UPDATE - I had a typo. Feel free to remove this message. ---
**Author:** ![bgwong](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/bgwong/32/262_2.png) [@bgwong](https://www.evolver.bio/u/bgwong)\ **Post date:** [April 15, 2021, 3:59pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/7 "2021-04-15T15:59:45Z")
Please continue thread on this post: > [@Failure to run calibration code, gets hung up at various points](https://www.evolver.bio/t/failure-to-run-calibration-code-gets-hung-up-at-various-points/165/): > > When trying to run the OD calibration code, the commands seem to get hung up before completion, but when initially setting up the machine a month ago the code was able to run fine. When trying to list calibration files, the terminal consistently gets hung up on ‘Waiting for evolver connection…’ When trying to run the actual calibration file, it either gets hung up after ‘connected to evolver as client’ or right bef… Please include screen shots of your terminal and also what IP address the evolver is. You can locate that on the upper right corner of the touch screen on the eVOLVER. ---
**Author:** ![zhou.g](https://avatars.discourse-cdn.com/v4/letter/z/b38774/32.png) [@zhou.g](https://www.evolver.bio/u/zhou.g)\ **Post date:** [January 12, 2024, 4:51pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/8 "2024-01-12T16:51:56Z")
Hi Brandon I am trying to download the calibrations.json file from the eVOLVER, but it seems that I did not use the right path, could you help me with this? ![屏幕截图 2024-01-12 113209](https://canada1.discourse-cdn.com/flex035/uploads/evolver/original/1X/ce5d7f57fcd2f912213d0c0dbad38f111c093491.png) Best, Guanyu ---
**Author:** ![Nate\_Borders](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/nate_borders/32/268_2.png) [@Nate\_Borders](https://www.evolver.bio/u/Nate_Borders)\ **Post date:** [January 25, 2024, 7:05pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/9 "2024-01-25T19:05:23Z")
Hi Guanyu, Try scp [pi@192.168.2.6](mailto:pi@192.168.2.6):/home/pi/evolver/evolver/calibrations.json calibrations.json You needed the full file folder path (/home/pi/evolver/evolver/) and the correct file name (calibrations.json) ---
**Author:** ![zhou.g](https://avatars.discourse-cdn.com/v4/letter/z/b38774/32.png) [@zhou.g](https://www.evolver.bio/u/zhou.g)\ **Post date:** [June 6, 2025, 7:11pm UTC](https://www.evolver.bio/t/calibration-files-in-evolver/108/10 "2025-06-06T19:11:39Z")
Sorry, I forgot to say thank you for one year, but still thank you, this is very helpful