# eVOLVER Comsumables

**URL:** <https://www.evolver.bio/t/evolver-comsumables/189>\
**Category:** Getting Started\
**Created:** [July 1, 2020, 12:55pm UTC](https://www.evolver.bio/t/evolver-comsumables/189 "2020-07-01T12:55:56Z")\
**Posts on this page:** 8\
**Page:** 1

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**Author:** ![bgwong](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/bgwong/32/262_2.png) [@bgwong](https://www.evolver.bio/u/bgwong)\
**Post date:** [July 1, 2020, 12:55pm UTC](https://www.evolver.bio/t/evolver-comsumables/189/1 "2020-07-01T12:55:56Z")

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Starting an eVOLVER experiment and figuring out what are consumables, or nice to haves in the lab? Here’s a list to get you started:

**Glass Culture Vessels - Chem Glass**

- [Vial Only, Sample, 40mL, Clear, 28x95mm, GPI 24-400, Pack of 144](https://chemglass.com/sample-vials-only-clear-borosilicate)
- [Fisherbrand™ Octagon Spinbar™ Magnetic Stirring Bars, 50 pack](https://www.fishersci.com/shop/products/fisherbrand-octagonal-magnetic-stir-bars-dia-0-12-in-length-0-5-in/1451357)

**Pipette Tips for Sampling (NOTE: Get the equivalent that works with your pipettors)**

- [Fisherbrand™ Extended-Length Tips (Lift Off Rack; 1 to 200 uL) Case of 3](https://www.fishersci.com/shop/products/fisherbrand-extended-length-tips-6/02681420?searchHijack=true&searchTerm=02681420&searchType=RAPID&crossRef=2681420&matchedCatNo=02681420)

**Tubing and Leur Connectors - McMaster-Carr**

- High-Temperature Silicone Rubber Tubing Semi-Clear White, Durometer 70A, 1/8" ID, 1/4" OD  
– 51135K73

- Chemical-Resistant Barbed Tube Fitting Tee Connector, for 1/8" Tube ID, 250°F Maximum Temperature  
– 5121K731

- Plastic Quick-Turn Tube Coupling, Plugs, for 1/16" Barbed Tube ID, Polypropylene  
– 51525K141

- Plastic Quick-Turn Tube Coupling, Plugs, for 5/32" Barbed Tube ID, Polypropylene  
– 51525K144

- Plastic Quick-Turn Tube Coupling, Sockets, for 1/16" Barbed Tube ID, Polypropylene  
– 51525K291

- Plastic Quick-Turn Tube Coupling, Sockets, for 5/32" Barbed Tube ID, Polypropylene  
– 51525K294

**Needles for interfacing with the culture ([see post for more details](https://www.evolver.bio/t/recommendations-for-septas-and-needles/59))**

- [Extra long Needles](https://www.air-tite-shop.com/p-73-air-tite-vet-premium-hypodermic-needle.aspx?variantid=901)\*\*
- [Premium Veterinary Hypodermic Needles, Sterile, Air-Tite, Length=3 (76 mm), Gauge=16](https://www.air-tite-shop.com/p-73.aspx?searchEngineName=air-tite-vet-premium-hypodermic-needle)
- [Silicone Septa](https://www.amazon.com/gp/product/B00822QBBA/ref=oh_aui_search_asin_title?ie=UTF8&psc=1)

**Networking**

- Computer w/ 8GB RAM (Mac or PC)
- Router ([Netgear recomended](https://www.evolver.bio/t/tutorial-configuring-local-router-and-computer-for-basic-evolver-use/71))

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**Author:** ![ericlyall](https://avatars.discourse-cdn.com/v4/letter/e/d2c977/32.png) [@ericlyall](https://www.evolver.bio/u/ericlyall)\
**Post date:** [January 12, 2021, 8:06pm UTC](https://www.evolver.bio/t/evolver-comsumables/189/2 "2021-01-12T20:06:20Z")

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What ID is the tubing that comes with the eVOLVER?

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**Author:** ![bgwong](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/bgwong/32/262_2.png) [@bgwong](https://www.evolver.bio/u/bgwong)\
**Post date:** [January 12, 2021, 8:19pm UTC](https://www.evolver.bio/t/evolver-comsumables/189/3 "2021-01-12T20:19:50Z")

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> **[McMaster-Carr](https://www.mcmaster.com/51135K11/)**
>
> McMaster-Carr is the complete source for your plant with over 595,000 products. 98% of products ordered ship from stock and deliver same or next day.

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**Author:** ![ericlyall](https://avatars.discourse-cdn.com/v4/letter/e/d2c977/32.png) [@ericlyall](https://www.evolver.bio/u/ericlyall)\
**Post date:** [January 14, 2021, 12:51am UTC](https://www.evolver.bio/t/evolver-comsumables/189/4 "2021-01-14T00:51:04Z")

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Can the tubes be autoclaved between experiments for sterility? Or do you recommend flushing them out?

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**Author:** ![bgwong](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/bgwong/32/262_2.png) [@bgwong](https://www.evolver.bio/u/bgwong)\
**Post date:** [January 14, 2021, 12:55am UTC](https://www.evolver.bio/t/evolver-comsumables/189/5 "2021-01-14T00:55:29Z")

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Please refer to our Jove paper

> **[Designing Automated, High-throughput, Continuous Cell Growth Experiments...](https://www.jove.com/t/59652/designing-automated-high-throughput-continuous-cell-growth)**
>
> The eVOLVER framework enables high-throughput continuous microbial culture with high resolution and dynamic control over experimental...

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**Author:** ![ericlyall](https://avatars.discourse-cdn.com/v4/letter/e/d2c977/32.png) [@ericlyall](https://www.evolver.bio/u/ericlyall)\
**Post date:** [January 19, 2021, 6:50pm UTC](https://www.evolver.bio/t/evolver-comsumables/189/6 "2021-01-19T18:50:51Z")

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Question about the pipette tips- we have LTS pipettors. Will [these tips](https://www.mt.com/ca/en/home/products/pipettes/bioclean-pipette-tip/packaging/racks/RT-LTS-A-250-X-768-8.html) work?

- I’m worried the OD is too wide (says the tips fit through 8mm ID septa holes). The sampling port is only ~4.4mm in diameter.
- I’m a little confused here, as the LTS pipette tips have a similar length and volume to the universal FisherBrand ones you have recommended, suggesting their diameter should be similar.
- Would you recommend buying these pipette tips?

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<div class="post-metadata">

**Author:** ![bgwong](https://yyz1.discourse-cdn.com/flex035/user_avatar/www.evolver.bio/bgwong/32/262_2.png) [@bgwong](https://www.evolver.bio/u/bgwong)\
**Post date:** [January 19, 2021, 7:03pm UTC](https://www.evolver.bio/t/evolver-comsumables/189/7 "2021-01-19T19:03:32Z")

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I’m not familiar with the Rainin tips/pipettes so I don’t have a specific recommendation here. Maybe someone else might have more experience.

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**Author:** ![yqlan](https://avatars.discourse-cdn.com/v4/letter/y/9e8a1a/32.png) [@yqlan](https://www.evolver.bio/u/yqlan)\
**Post date:** [November 8, 2022, 12:20am UTC](https://www.evolver.bio/t/evolver-comsumables/189/8 "2022-11-08T00:20:17Z")

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Hi there,

Did you try these tips you mentioned? Can they pass through the sampling holes with ~4.4mm in diameter?

Thanks.
