# Switching steering off during OD measurement

**URL:** <https://www.evolver.bio/t/switching-steering-off-during-od-measurement/470>\
**Category:** Software\
**Created:** [July 25, 2023, 9:50am UTC](https://www.evolver.bio/t/switching-steering-off-during-od-measurement/470 "2023-07-25T09:50:19Z")\
**Posts on this page:** 1\
**Showing post:** 2

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**Author:** ![ezirayw](https://avatars.discourse-cdn.com/v4/letter/e/dfb087/32.png) [@ezirayw](https://www.evolver.bio/u/ezirayw)\
**Post date:** [July 27, 2023, 6:44pm UTC](https://www.evolver.bio/t/switching-steering-off-during-od-measurement/470/2 "2023-07-27T18:44:45Z")

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Hopefully this helps:

> [@At lower culture volumes optical density readings are affected by stirring](https://www.evolver.bio/t/at-lower-culture-volumes-optical-density-readings-are-affected-by-stirring/367/2):
>
> I think the vortex from the stir is the main thing causing the issue here. If you need lower volumes in the standard eVOLVER culture vial with OD measurements, do not go below 10 mL and be sure to turn the STIR off for ~20s before taking an OD measurement to allow enough time for the SAMD21s to collect clean data. Thanks for the write up! It’s great to have these details documented.

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