I’ve been running an experiment for ~850 hours, and for the last ~100 hours about half of the vials stopped back-diluting. I had not touched the machine for over 50 hours at that point. I’ve turned off-and-on the turbidostat and router, and restarted the python script multiple times, but only one of those vials began back-diluting again. Has anyone encountered this? If so, do you know what might have gone wrong/what helped you fix the problem? I’m totally stumped.
P.S. This question is unrelated to my question about media tubing hardware posted a week ago, since I haven’t yet implemented the change in media containers from that post.