Hi, eVOLVER experts
I am now setting some experiments, and found that I need 25 L for almost 5 days experiments ! (20 mL for culture volumes, 1.5 h for Avg.doubleing time, 16 for the number of cultures)
I calculated the required media by using the metrics in the Supplementary Notes 8 below.
Because it’s my first time with continuous culture, it is just surprising me.
Is 25 L for 5 days kind of normal volumes often used ??
Thank you very much for your help!