Question about best practice for a media change to a container with long tubing:
I’m planning to connect my ongoing experiment with a larger media container (40L carboy). The tubing is several feet, since the carboy rests on the floor. When I change the media, all that tubing will be full of air. After that I could:
- Just connect the vial, and let the pumps try to dilute until media has been brought all the way through the tubing. Pro: automatic. Con: seems like this could mess with the growth rate by being logged as separate pump events due to the intake time of >20s.
- Connect the vial and then run the fluid_test script to pull media up till it is near the vial. Pro: won’t be logged as separate pump events within the script. Con: risks overflowing the vials if I estimate the pump time wrong.
Has anyone tried changing media to containers with long tubing before? And/or Brandon do you have advice?