eVOLVER Comsumables

Getting Started
1

Starting an eVOLVER experiment and figuring out what are consumables, or nice to haves in the lab? Here’s a list to get you started:

Glass Culture Vessels - Chem Glass

Pipette Tips for Sampling (NOTE: Get the equivalent that works with your pipettors)

Tubing and Leur Connectors - McMaster-Carr

  • High-Temperature Silicone Rubber Tubing Semi-Clear White, Durometer 70A, 1/8" ID, 1/4" OD
    – 51135K73

  • Chemical-Resistant Barbed Tube Fitting Tee Connector, for 1/8" Tube ID, 250°F Maximum Temperature
    – 5121K731

  • Plastic Quick-Turn Tube Coupling, Plugs, for 1/16" Barbed Tube ID, Polypropylene
    – 51525K141

  • Plastic Quick-Turn Tube Coupling, Plugs, for 5/32" Barbed Tube ID, Polypropylene
    – 51525K144

  • Plastic Quick-Turn Tube Coupling, Sockets, for 1/16" Barbed Tube ID, Polypropylene
    – 51525K291

  • Plastic Quick-Turn Tube Coupling, Sockets, for 5/32" Barbed Tube ID, Polypropylene
    – 51525K294

Needles for interfacing with the culture (see post for more details)

Networking

2

What ID is the tubing that comes with the eVOLVER?

3
4

Can the tubes be autoclaved between experiments for sterility? Or do you recommend flushing them out?

5

Please refer to our Jove paper

6

Question about the pipette tips- we have LTS pipettors. Will these tips work?

  • I’m worried the OD is too wide (says the tips fit through 8mm ID septa holes). The sampling port is only ~4.4mm in diameter.
  • I’m a little confused here, as the LTS pipette tips have a similar length and volume to the universal FisherBrand ones you have recommended, suggesting their diameter should be similar.
  • Would you recommend buying these pipette tips?
7

I’m not familiar with the Rainin tips/pipettes so I don’t have a specific recommendation here. Maybe someone else might have more experience.

8

Hi there,

Did you try these tips you mentioned? Can they pass through the sampling holes with ~4.4mm in diameter?

Thanks.