Pump in from multiple sources

sophie9499 · June 22, 2021, 9:30pm

Hi people,

I’m trying to pump in from two sources (different amount) into one vial so I wonder if it’s possible and if you’ve got it to work before, I’d really appreciate any suggestions and advice!

Thanks!

bgwong · June 22, 2021, 10:44pm

This should be fairly straight forward by controlling two separate peristaltic pumps, each from its own media source. If you have any other more specific questions, please let me know.

Brandon

sophie9499 · June 22, 2021, 10:55pm

Thank you for your quick reply! Just wondering how to implement it in terms of coding. Could you give an example of how to execute this in python? Thanks!

bgwong · June 23, 2021, 3:06pm

This would depend on how you are trying to set it up, so I can’t really give you specific guidance unless you share what your setup looks like.

Generally, if you want a reference, the fluidic commands are executed here:

github.com

FYNCH-BIO/dpu/blob/master/experiment/template/custom_script.py#L147

    
      
                              file_path = os.path.join(save_path, EXP_NAME, 'pump_log', file_name)
          
          
                    text_file = open(file_path, "a+")
                              text_file.write("{0},{1}\n".format(elapsed_time, time_in))
                              text_file.close()
                  else:
                      logger.debug('not enough OD measurements for vial %d' % x)
          
          
    # send fluidic command only if we are actually turning on any of the pumps
              if MESSAGE != ['--'] * 48:
                  eVOLVER.fluid_command(MESSAGE)
          
          
        # your_FB_function_here() #good spot to call feedback functions for dynamic temperature, stirring, etc for ind. vials
              # your_function_here() #good spot to call non-feedback functions for dynamic temperature, stirring, etc.
          
          
    # end of turbidostat() fxn
          
          
def chemostat(eVOLVER, input_data, vials, elapsed_time):
              OD_data = input_data['transformed']['od_90']
          
          
    ##### USER DEFINED VARIABLES #####

The logic for determining which pump to actuate is just above that portion of the script. The setting is passed through the MESSAGE variable to the fluid_command function in the eVOLVER library:

github.com

FYNCH-BIO/dpu/blob/master/experiment/template/eVOLVER.py#L255

    
      
                      'immediate': immediate, 'recurring': True}
              logger.debug('stir rate command: %s' % data)
              self.emit('command', data, namespace = '/dpu-evolver')
          
          
def update_temperature(self, temperatures, immediate = False):
              data = {'param': 'temp', 'value': temperatures,
                      'immediate': immediate, 'recurring': True}
              logger.debug('temperature command: %s' % data)
              self.emit('command', data, namespace = '/dpu-evolver')
          
          
def fluid_command(self, MESSAGE):
              logger.debug('fluid command: %s' % MESSAGE)
              command = {'param': 'pump', 'value': MESSAGE,
                         'recurring': False ,'immediate': True}
              self.emit('command', command, namespace='/dpu-evolver')
          
          
def update_chemo(self, data, vials, bolus_in_s, period_config, immediate = False):
              current_pump = data['config']['pump']['value']
          
          
    MESSAGE = {'fields_expected_incoming': 49,
                         'fields_expected_outgoing': 49,

I would recommend playing around this to familiarize yourself with a small test script. For example, the following includes test stir and temp commands. You could easily implement something similar to test fluidic commands.

github.com

FYNCH-BIO/dpu/blob/master/experiment/server_test.py

import socket
from socketIO_client import SocketIO, BaseNamespace
import asyncio
from threading import Thread
import random
import time

STIR_VALUES = [[0] * 16, [8] * 16] # flip between on and off
TEMP_VALUES = [4095] * 16 # always off

EVOLVER_IP = '192.168.1.8'
EVOLVER_PORT = 8081
evolver_ns = None
socketIO = None

class EvolverNamespace(BaseNamespace):
    def on_connect(self, *args):
        print("Connected to eVOLVER as client")

    def on_disconnect(self, *args):

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