Calibration Files in eVOLVER

[bgwong]

The od_90 or od_135 refers to the angle the photodiode is to the LED, not the wavelength of light. You have to type in od_90 or od_135 to calibrate those respective photodiodes.

Below is a thread on why we have two photodiodes.

OD measurements with two photodiodes?

Summary I’m looking for some advice on how to properly do calibrations with two variables. Background I’m trying to integrate hardware configuration for OD measurements to improve the consistency and dynamic range of the sensors. I figured adding a different sensor at a different LED-diode offset would do the trick. I did a simple, rough experiment and collected measurements off of 90 degree and 135 degree offsets with increasing amount of milk (to approximate scattering from cells). Heres t…